You can use decreasing axes by flipping the normal order of the axis limits
import matplotlib.pyplot as plt
import numpy as np
t = np.arange(0.01, 5.0, 0.01)
s = np.exp(-t)
plt.plot(t, s)
plt.xlim(5, 0) # decreasing time
plt.xlabel('decreasing time (s)')
plt.ylabel('voltage (mV)')
plt.title('Should be growing...')
plt.grid(True)
plt.show()